Commercial production of poultry meat and eggs would be impossible without disease control. In this review, the author looks back at more than 50 years of close communication between the Lohmann Veterinary Laboratory and international experts to apply the latest scientific and technical knowledge in disease prevention.
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Keywords: poultry diseases, Newcastle, Fowl Pox, AE, IB, Marek’s, Leukosis, Gumboro, CAV, Salmonella, ectoparasites, free range management, Avian Influenza
With the development of the modern poultry industry, several poultry diseases spread due to vertical transmission and caused high mortality. Mycoplasma gallisepticum (Mg), a trigger of E. Coli, caused substantial losses on broiler farms. Pullorum and Leucosis virus types A in layers and type J in broilers had to be eradicated from breeding stock to stop vertical transmission. New diseases spread during the last decades: In 1969, Biggs identified the cause of the tumor form of Marek’s Disease and, together with Witter, developed vaccines based on attenuated Marek herpes virus (Biggs) and turkey herpes virus FC 126 (Witter). The CVI 988 (Rispens) virus has been used since 40 years and is still effective. V. Bülow and Vielitz identified the chicken anemia virus in 1983 as the cause of anemia in broiler flocks. EDS virus and other types of adeno virus were identified as causes of mortality and depressed production and egg quality. Vaccines to control Salmonella infections with S. typhimurium and S. enteritidis were developed and are widely used to reduce the risk of food poisoning.
The re-introduction of free range management and the ban on effective drugs to control diseases like Histomoniasis and necrotic enteritis brings us back to disease problems of the 1960s.
The evolution of avian pathology during the past five decades is closely linked to two major developments in global poultry production:
1. Genetic selection of meat-type and eggtype chickens and other poultry species
2. Development of housing and management for efficient production of poultry meat and eggs in large units
Nowadays commercial white egg and brown egg layers produce more than 300 eggs per year and consume about 120 g of feed per egg, roughly 50% less than 50 years ago. Total egg mass per hen often exceeds 20 kg per year, ten times the hen’s body weight. Similarly, meat type chickens are reaching market weight much earlier and on less feed than 50 years ago, in Germany e.g. 2 kg live weight at 35 days of age on less than 1.6 kg feed per kg gain. Before specialized meat-type and egg-type chickens were imported from North America in the 1950s, laying hens laid about 120-150 eggs per year, and it took cockerels 4 months to reach 1 kg live weight as “broilers”. The first imports from North America produced almost twice the number of eggs or grew twice as fast, with corresponding savings in feed cost.
Free range management, common in the 1950s, was associated with high mortality rates from predators and numerous parasitic and bacterial infections.
While studying veterinary medicine, my knowledge of chicken diseases was limited to pullorum disease, coccidiosis and
Marek‘s Disease. When I started to work for Heinz Lohmann in 1959, the first practical lesson to learn was that birds in free range management had to be caught from trees for pox vaccination!
To prevent high mortality due to free range management, environment controlled chicken houses were built in the 1950ies,
and the production of poultry meat and eggs became a full-year business focused on utilization of facilities. As a result, mortality was lower, weight gain and feed efficiency improved.
To benefit from advancements in poultry science in North America during WW II, Lohmann had signed agreements with
two American primary breeders, Nichols (1956) and Heisdorf & Nelson (1958) to breed and distribute broilers and laying
hens, respectively, in Germany and subsequently in Europe and world-wide. With these contracts, American know-how in
applied poultry science was also imported.
In 1960 we had the first severe outbreaks of ND in Germany, with 30-40% mortality in broiler flocks. Only inactivated ND vaccines existed in Germany in those days, requiring individual injection. We learned of a live Newcastle virus vaccine in the
United States, based on the Hitchner B1 lentogenic virus that could be administered in the drinking water. Importing and
applying this vaccine soon brought the disease under control. As a result of these experiences we became convinced that
only live vaccines are powerful enough to protect the birds sufficiently.
In the early 1960ies fowl pox disease was also detected in Germany. At that time a live vaccine of the Behringwerke based on an attenuated fowl pox virus strain proved to be very successful and the disease disappeared for a long time. Unfortunately, the awareness of fowl pox risks also vanished and live vaccination against fowl pox was eliminated from the vaccination schedule for many years. The disease appeared again in 1999, with more than 60 flocks affected in Germany, and live pox vaccination was re-introduced successfully.
In the early 1960ies breeding stock of layers and broilers were generally kept in well isolated houses with best possible hygienic conditions. These flocks remained free of a number of natural infections duringrearing. However, these infections could be introduced during hatching egg production, after the birds were transferred to laying houses. This resulted in 1961 in the first epidemic disease in unprotected broiler progeny. The chicks showed paralysis and tremor, and the condition was diagnosed as avian encephalomyelitis (AE), also called epidemic tremor. This is a viral infection, transferred vertically from the mother via the hatching egg to the offspring.
Since American poultry consultants like Dr Donald Zander (H&N-Laboratory, USA) had already had experience with this disease, diagnostics and preventive treatment were quickly introduced. Parent birds were prevented from shedding the
AE-virus before they started to produce hatching eggs. Brains of symptomatic chickens were homogenized and administered
to the parent birds via the drinking water during rearing. This was the first autogenous vaccine we produced!
Feed born encephalomalacia due to insufficient supply of vitamin E and antioxidants was diagnosed as a major problem
in broilers. This condition disappeared when the feed formulation was corrected. Another real problem was the fatty liver
syndrome, typically found in high producing caged laying hens. In collaboration with Prof. Köhler (10) from the pathology
institute in Vienna we were able to identify feed composition as a major cause. The incidence was substantially reduced by
replacing carbohydrate energy by fat (unsaturated fatty acids) in feed formulation.
With the introduction of laying cages in the early 1960ies the stocking density per unit and the general bird density in
certain regions also increased. This apparently contributed to more and more cases of IB in laying hens shortly after the
AE-epidemic was solved. A large number of vaccination trials were carried out between 1963 and 1964, using attenuated IB strains of the American Massachusetts type of virus (H120, H52). These vaccine strains protected sufficiently well against
the German field strains, whereas in the Netherlands variant virus strains of IB such as IB 274 and IB 1466 were isolated. IB-vaccines were commonly attenuated and manufactured on chicken embryos. A negative impact was the presence of maternal antibodies against IB-virus within the eggs that caused the virus yield to be low. This situation was significantly improved, when eggs free of specified antibodies and antigens (VALO eggs) were introduced (26).
In the course of the 1960s a new form of Marek’s disease occurred in southern Europe. Until then we had known only the
classical form causing paralysis and iridocyclitis. With the new form the birds developed tumours in organs, and mortality
was extremely high. In Spain up to 50% of the chickens died. As a consequence a number of chicken breeders intensified
resistance breeding. Geneticists of H&N and Lohmann decided to split the odds and selected parallel lines (i) only for conventional traits, (ii) only for MD resistance and (iii) on an index with similar emphasis on egg production and MD resistance. The results of cumulative genetic progress in 5 years were published by Flock et al. (8) and showed a reduction of MD mortality by 20% in the MD selected sub-lines vs. 2 kg increased egg mass in the main lines. The competition between geneticists and veterinarians to control this disease was decided when the virus was identified as a herpesvirus by Dr Biggs in 1969 and Dr Witter in 1970 found the HVT virus in turkeys to be non-virulent and protective against Marek’s Disease in chickens. The first European HVT vaccine against Marek’s Disease was introduced into Spain in 1971 (1, 20, 35).
In The Netherlands Dr Bart Rispens used a non-pathogenic Marek herpesvirus he had isolated from chickens, as a vaccine strain. He called this virus CVI 988 (Central Veterinary Institute). At that time we in Germany were satisfied with the HVT based vaccine (22, 23).
At the end of the 1970ies in southern Europe and North Africa the first cases of Marek’s Disease were claimed despite
HVT vaccination. Especially in Egypt this resulted in great losses. It was striking that the bird shipments from Holland did not develop the disease. These birds had been vaccinated with the Rispens strain CVI 988 in the Dutch hatcheries. We quickly switched from HVT to the Rispens vaccine and thus solved the problem (5, 8, 9, 11, 17, 22, 23, 25, 27, 29).
In 1967, the first cases of Gumboro disease occurred in white layers in Germany, characterized by low mortality rates (18).
In 1976, in The Netherlands, a new disease called Egg-drop syndrome (EDS) occurred, the cause of which was a Duck adenovirus. This virus was introduced through contamination of duck fibroblast cultures used in Marek vaccine production in The Netherlands. The Lohmann vaccine remained free from it because the vaccine was prepared in primary SPF chicken embryo fibroblast cultures.
In the late 1970s Prof. Becht and his research student Mrs. Cursiefen from the University of Giessen described a mutant
of the virulent Gumboro strain Cu-1. The Cu-1M virus was a mini-plaque variant and as such was non-pathogenic but very invasive. In 1978 Lohmann developed a live Gumboro vaccine using this virus strain Cu-1M (6, 7).
In the early 1980s layer flocks suffered from losses of up to 70% caused by so called vv-IBDV. These field strains can break through much higher maternal antibody levels than so called intermediate Gumboro vaccines. Therefore it became necessary
to vaccinate birds earlier with stronger live IBD-vaccines (28).
Ever since I worked with poultry, avian mycoplasmosis was an issue. Especially in the presence of management stress
or other respiratory pathogens broilers showed depressed weight gain and layers a significant drop in egg production
due to Mycoplasma gallisepticum. Subsequent Coli-infections caused heavy losses. Therefore the permanent aim of the global poultry industry was the eradication of this microorganism. Well managed farms were completely depopulated, extremely thoroughly cleaned and disinfected before new birds were placed. To remove the mycoplasmas from the hatching eggs, tylosin was injected on the 9th day of incubation. This enabled us to eradicate MG infections from our broiler breeders in 1968/69 (16). In contrast, our layer breeders were infected with tylosin-resistant MG. These Mycoplasms were non-pathogenic, but the birds became serologically positive. The eradication succeeded later by in-ovo injection of Baytril. Mycoplasma synoviae (MS) is a very contagious microorganism and is still found in many farms. For a long time we could not verify any pathogenicity, but more recent evidence indicates that MS is responsible for low egg shell quality (“apex”) and respiratory problems.
In 1980/81, when infectious laryngotracheitis (ILT) occurred in Germany, we developed a vaccine based on embryonated
eggs. This vaccine proved to be extremely effective, but possessed a certain residual pathogenicity. This was probably the
reason for the good efficacy. The residual pathogenicity only came up when the vaccine was administered via the drinking
water. The same virus individually administered by eye drop, caused no losses.
In Bavaria a new disease in broilers occurred in 1982, diagnosed as gangrenous dermatitis. It was noticed that always the
offspring of young parents was affected. It soon became clear that vertical spread was going on. The parents became infected in the beginning of the egg production period due to contact with staff and equipment from hatcheries. In the rearing period they had remained seronegative, because they were very well isolated. So it was obvious that the suspected pathogen should already be administered during rearing in order to develop a protective immunity before the egg laying period started.
The seroconversion of the parents would have to provide protective antibodies to the offspring and at the same time stop
the horizontal and vertical spread of field virus. The causative agent was unknown to us. We suspected a virus. So we transferred litter of older flocks whose progeny already showed this disease to young flocks. This of course contradicted the
general rules of hygiene, but solved the problem. We then started a research program. Together with the Free University
of Berlin and Prof. von Bülow we worked intensively on the viral culture, and he was able to identify and propagate in cell culture the chicken anemia virus (CAV). We discovered that even our SPF-flocks were infected. Thus our SPF-flocks possessed maternal antibodies. Transmission of the disease (3rd Koch‘s postulate) to SPF animals could therefore not succeed. By working with Mr. von Bülow, we finally were able to produce CAV free SPF-flocks. These were the first flocks worldwide possessing the status „CAV-free“. In the further course of our project we were able to reproduce Chicken anemia in SPF embryos and eventually produced a live vaccine. We called the vaccine „Thymovac“ because the chicken anemia virus atrophied the thymus besides affecting the bone marrow. This vaccine was licensed in Germany in 1990 and was the first live CAV chicken vaccine worldwide (2, 3, 4, 21, 30, 31, 32).
The epidemiology of anemia virus was thus similar to that of the AE and the adenovirus infection: The infection took place
during egg production. The virus is transmitted vertically, followed by disease of the chicks in the first weeks of life. I saw serious cases of adeno virosis in Kuwait with up to 40% losses in chicks hatched from imported Dutch hatching eggs, carrying the disease.
In 1983 increasing cases of viral arthritis occurred in broiler flocks due to Reo virus infections. This happened although their
parents were vaccinated with conventional inactivated Reo virus vaccines. We isolated variant Reo viruses, which were then
typed by Prof. van der Heide. In order to gain protection in the progeny we developed inactivated Reo vaccines from these
variants for parent vaccination.
From 1985, we could differentiate for the first time serotype specific antibodies by means of micro-IB-SN-tests. The development of this test was part of the doctoral thesis of M. Voß at the Free University Berlin (33). The test allowed the serological differentiation of IB virus variant infections.
In the late 1970s we intensified the screening of all egg lines at the pedigree level to eliminate all virus shedders. This eradication apparently contributed significantly to the fact that entries of commercial LSL layers from different parent flocks
averaged more than 300 eggs per hen housed to 500 days of age in German random sample tests. The eradication of
ALV from primary breeder stocks was an essential prerequisite for the introduction of feather-sexing White Leghorn crosses.
The disease almost disappeared in commercial layers. Leukosis became important once more in broiler breeders in the early
1990s (ALV-J); the virus strain was eradicated within two years.
In 1987 progeny of broiler breeders suffered from high losses due to inclusion body hepatitis (IBH) and hydropericardium. At
the same time a similar outbreak of the same disease was observed in Pakistan, which was called Angara Disease. As a
causative agent an Adeno virus could be isolated from infected progeny. Again the progeny was protected, when parent birds
had seroconverted before beginning of lay. The disease was transmitted vertically. Therefore, the Adeno virus was amplified
and a live vaccine was administered to the next parent bird generation during rearing via the drinking water.
In 1989 the British Minister of Health Edwina Currie declared: “We do warn people, that most of the egg production in this
country, sadly, is now infected with Salmonella”. Also in Germany it was a big threat to the poultry industry in the 90ies when Salmonella infections of poultry resulted in Salmonella outbreaks in man. The causative agents were Salmonella enterica enteritidis and Salmonella enterica typhimurium. In Germany live Salmonella vaccinations of poultry were introduced based on research of Prof. Linde in Leipzig. The vaccination against Salmonella in chicken layers became obligatory in Germany in 1994. Only a few years later the Salmonella vaccinations were also introduced in the UK. The Salmonella metabolic drift mutants of Prof. Linde were a great success, resulting in a significant reduction in the prevalence of Salmonella in poultry products and in human outbreaks (12-14).
In the 1990s red mites (Dermanyssus gallinae) became an increasing problem, especially among densely housed layers.
Reduced egg production, nervousness of flocks and increasing mortality, up to 30%, were the consequences. This problem is unsolved until today. Cleaning, thorough washing and the application of pesticides is only reducing the problem. Intensively
housed broilers on the other hand can be accompanied with black beetles (Alphitobius diaperinus), that appear in billions in
the litter. Both ectoparasites can harbour a number of different bacterial and viral infectious agents. The parasites and with
them a number of diseases spread from flock to flock.
Despite the negative experience with free range management in the late 1950s, free range housing was introduced again in
the 1990s. Advantages and disadvantages of different housing conditions of layers are compared in the following table.
As shown in this summary, cage management is preferred in terms of health and product quality, but does not meet animal
welfare demands and has therefore been banned in some countries. Floor husbandry provides better conditions in terms of
bird welfare, but at the expense of product quality and animal health.
Many bacterial infections such as E.coli, Pasteurella and Erysipelothrix re-occurred under free range conditions. Also Ornithobacterium rhinotracheale as a respiratory disease agent and parasites such as Histomoniasis have been detected. Among those bacteria a number of isolates have been found that cannot be typed by conventional methods. Therefore no registered vaccines are on the market and autogenous inactivated vaccines currently fill these gaps.
Autogenous vaccines are also increasingly used since the overuse or misuse of antibiotics has been linked to the emergence and spread of antibiotic resistant micro-organisms. Their treatment is ineffective, and they pose a serious risk to public and animal health. Autogenous vaccines have become a real alternative to antibiotics. Well known examples of antimicrobial resistant micro-organisms among the chicken population are multi-resistant Staphylococcus aureus and E. coli.
Regarding the use of antibiotics in livestock the old paradigm is still applied today: “If the microbes are exposed over time to a suboptimal concentration of antibiotics, they defend themselves by developing resistance. Therefore attack bacteria with a high dose of antibiotics over a short time.” However this is questioned today, since it only seems to be true in-vitro, but not in-vivo (24). In-vivo it may well be just the other way round. In the animal, low doses given over a long period of time allow the healthy flora to develop again, which it doesn’t do under high antibiotic concentrations.
|Spalte 1||Conventional Cages||Enriched cages||Aviary||Barn||Free Range|
+ + +
- - -
+ + +
+++ = best;
+ + +
- - - = worst
The first description of avian influenza dates back to 1878 by Perroncito in northern Italy. Highly pathogenic avian influenza
(AI) virus known as H5N1 was discovered in 1997 on a goose farm in China. Since 1997 H5N1 has spread to over 40
countries in Asia, Africa and Europe. Severe outbreaks occurred in the beginning of the new millennium in Italy, then in The
Netherlands and Germany. The virus is endemic in wild water fowl and can spread to domestic birds.
In the case of low pathogenic strains such as H9N2 birds often only shed the virus without any clinical symptoms. The virus
can even spread to humans or other mammals. Highly pathogenic AI viruses are a permanent threat to the poultry industry,
because in case of outbreaks the whole flocks are killed (stamping out policy in the EU). In Europe vaccination against highly pathogenic AI viruses (H5, H7) is banned. A number of research projects based on genetically modified live AI-viruses failed due to the very frequent genetic shift and drift of the virus. At the moment the vaccine industry has declared the inactivated AI vaccines to be the gold standard against the disease.
During past decades, general hygiene, biosecurity and vaccinations contributed to a significant reduction of mortality from
a range of poultry diseases. Adapted to local disease risks, these principles remain essential for disease control. The methods will be further refined, and the Veterinary Laboratory of Lohmann Tierzucht will continue to play a significant role in the application and promotion of new knowledge.
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I thank Brigitte Othmar for the support in the preparation of this paper. Slides presented at the Meeting in Evora, Portugal, may be obtained from the author
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